Ultimele Noutati
Workshop SheepNet
 
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09
Postat de: Admin ZOO
Sectiune: Fac. de Zootehnie si Biotehnologii
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TE-2012-3-0092 Rezultate Etapa II/2014

 Virulence characteristics of five new Campylobacter jejuni chicken isolates

 
Lavinia Stef, Ada Cean, Aida Vasile, Calin Julean, Dan Drinceanu and Nicolae Corcionivoschi
 
Abstract
 
Campylobacter enteritis has emerged as one of the most common forms of human diarrheal illness. In this study we have investigated the virulence potential of five new C. jejuni chicken isolates (RO14, RO19, RO24, RO29 and RO37) originated from private households in the rural regions of Banat and Transylvania in Romania. Following isolation and in vitro virulence assay, on HCT-8 cells, our results show that all the C. jejuni chicken isolates overcome the virulence abilities of the highly virulent strain C. jejuni 81-176. Motility, an important virulence factor was significantly improved in all the new chicken isolates. The ability to survive to the antimicrobial activity of the human serum, to resist to the violent attack of bile acids and to survive in the presence of synthetic antibiotics was increased in all the chicken isolates. However, these were statistically significant only for isolates RO29 and RO37. In conclusion our study shows, based on invasiveness and motility, and also on the data provided by the serum and bile resistance experiments that all the new chicken isolates are able to infect human cells, in vitro, and could potentially represent a health hazard for humans.
 
 
 
Lactic acid bacteria liophilisate for use as probiotic in poultry feed
 
Calin Julean, Lavinia Stef, Dan Drinceanu, Ada Cean, Monica Dragomirescu, Teodor Vintila, Voichita Gherasim, Nicolae Corcionivoschi

Abstract 

The purpose of the paper is to apply liophilisation techniqueof four strains of lactic acid bacteria to be used as probiotic in poultry feed. In our experiment we used the following strains of lactic acid bacteria: Lactobacillus paracasei CMGB 18, Lactococcus lactis CMGB 33, Lactobacillus rhamnosus CMGB 34, Lactococcus lactis CMGB 32. The strains were preserved by freezing at - 60ºC. After the freeze-drying, with protectant based on skim milk with MRS - broth we obtained liophilisates with cell concentration in the range of 1·109 CFU/g. These products can be used as probiotics in poultry feed in concentration of 1 g/kg combined feed.

 
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