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TE-2012-3-0092
 THE ROLE OF PROBIOTIC BACTERIA IN PREVENTING CAMPYLOBACTER JEJUNI COLONIZATION OF POULTRY
Tip: TE-2012-3-0092, perioada 2013-2015
This work is supported by a grant of the Romanian National Authority for Scientific Research, CNCS –UEFISCDI, project number PN-II-RU-TE-2012-3-0092.
Project director
Eng. Nicolae Corcionivoschi PhD
 
Research team
Prof. Eng. Lavinia Ștef PhD
Prof. Eng. Dan Drînceanu PhD
Eng. Cean Ada PhD
Eng. Cãlin Julean PhD
 
Project Summary
Campylobacter is the leading cause for gastroenteritis in humans, the major clinical signs is bloody diarrhea and in severe cases can alter the integrity of the nervous system. The main cause for contamination is contaminated poultry meat. At birds C. jejunii is not crossing the intestinal barrier colonizing only the intestinal mucous. In the last decade the Europeran Union has forbidden the usage of antibiotics in order to prevent the contamination of poultry meat due to the persistent effects after treatment. Probiotics are known as efficient is pathogen bacterial elimination from intestines, by occupying the sites which are normally taken by the pathogens. Our preliminary studies confirmed the hypotheses that probiotics are efficient in preventing the colonization of the epithelial mucosa with C. jejunii. These strains also have antimicrobial activity against C. jejuni. Tis grant is perusing the effect of the probiotic bacteria on preventing the colonization of intestinal mucosa with C. jejunii in poultry. In vitro and in vivo studies will be performed in order to test combinations of probiotic bacteria that are efficient in preventing C. jejunii colonization. The final aim of this study is to create a probiotic product that is efficient in reducing or elimination from the digestive tract of C. jejunii in poultry.
 
Project objective
The main objective of the project is isolation of Campylobacter jejuni stains from West part of Romania. After the isolation and identification of the stains, they will be tested for virulence and compared with the already known strains. We will also test the inhibitory effect of probiotic strains and their use in preventing C. jejuni colonization in farm poultry
Estimated results
 
1. Identification of Campylobacter jejuni in the digestive tract of broilers raised in different farms in the western part of Romania. Characterization for the first time, of the C. jejuni strains present is the industrial and family farms from the West part of Romania. These strains will be testes for virulence by using in vitro virulence studies on human epithelial cells.  
2. Identification of probiotic strains with inhibitory effect by preforming in vitro virulence tests. These tests will be performed by using human epithelial cells which were exposed to probiotic strains.
3. Using probiotic stains in poultry forage and testing it’s inhibitory effect for C. jenunii colonization.
4. Investigating the probiotic effects in contamination of the poultry carcasses.
5. Probiotic product that is efficient in reducing or elimination from the digestive tract of C. jejunii in poultry in the West part of Romania as well in industrial and family farms.

Objectives and activities 

 Results obtained phase I /2013 

 Results obtained phase II /2014 

 
Tip: TE-2012-3-0092, perioada 2013-2015

Updated by: Admin ZOO on 2015-12-03
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